Establishment of anticancer drug taxol detection of thin-layer chromatography ( TLC ) method, can be used for rapid screening of taxol producing strains. The paclitaxel standard or endophytic fungus fermentation liquid extract, point to m10-40particle size silica gel thin layer chromatography plate, with chloroform - methanol liquid chromatography as the expansion agent, and then immediately use containing1% vanillin sulphuric acid liquid spray color, study on paclitaxel characteristic blue spots. Taxol separation detection results, with the aid of taxol by thin layer chromatography and color reaction can be filtered to produce taxol endophytic fungi. Application of thin layer chromatography technique on endophytic fungi fermentation products were detected, which can conveniently, accurately to the screening of taxol producing strains, and strains can be speculated that taxol producing ability; to produce taxol medicinal fungi screening study, more efficient than HPLC method.
也就這樣了，太亂了